Accuris AccuFi High Fidelity Master Mix, 2x conc., 200 reactions

AccuFi™ High Fidelity, Hot Start Master Mix (Series PR1002-HFHS)

• Ultra-high fidelity: Exhibits highly accurate DNA synthesis supported by coupled 5’→3’ DNA polymerase activity and intrinsic 3’→5’ exonuclease proofreading.
• Hot Start DNA polymerase: minimizes nonspecific amplification, reduced background and enhances reproducibility in PCR.
• Strong amplification performance: Supports efficient amplification across challenging templates, including GC-rich sequences, low-quality, and long-range targets, while maintaining high yield.
• Fast extension rate: Delivers rapid DNA synthesis at approximately 1 kb/10 seconds, supporting accelerated PCR cycling.

Accuris AccuFi HFHS PCR Mix is optimized 2X master mix that includes AccuFi High Fidelity polymerase, a proprietary reaction buffer system, MgCl2, and ultra-pure dNTPs. The engineered polymerase exhibits rapid extension kinetics (~10 s/kb), enabling efficient fast cycling protocols while maintaining high processivity. The formulation supports robust long-range amplification, with reliable performance on templates exceeding 15 kb. The enzyme possesses both 5’→3’ DNA polymerase activity and intrinsic 3’→5’ exonuclease proofreading activity, resulting in ultra-low error rate (below the detection limit) compared to Taq DNA polymerase. The high fidelity of the enzyme supports applications where sequence integrity is critical, such as molecular cloning, next-generation sequencing (NGS) library construction, and site-directed mutagenesis. The optimized buffer system enhances amplification efficiency across challenging templates, including GC-rich and partially impure DNA samples, while maintaining high yield and specificity with minimal reaction optimization.

Manufacturer Part Number/SKU: PR1002-HFHS-200-EA

Tags: PCR